TB-500 is a synthetic peptide corresponding to the actin-binding domain of Thymosin Beta-4 (Tβ4), a naturally occurring protein involved in cell migration, wound healing, and tissue regeneration. It is sold as a lyophilized powder and requires reconstitution with bacteriostatic water before injection. TB-500 is dosed in milligrams rather than micrograms, which affects how you set up your concentration.
What You Need
- TB-500 vial (commonly 2 mg or 5 mg)
- Bacteriostatic water (BAC water) for injection
- U-100 insulin syringes (0.5 mL or 1 mL, 28-31 gauge)
- Alcohol swabs
- Refrigerator at 36-46°F (2-8°C)
Concentration Selection for TB-500
TB-500 is typically dosed at 2-10 mg per week in research protocols. Because the doses are in the milligram range, you can use a relatively high concentration without creating impractically large injection volumes. A 1 mg/mL concentration is common and gives draw volumes in the 20-100 unit range depending on dose.
- 2 mg vial + 2 mL BAC water = 1 mg/mL (40 units per 2 mg dose)
- 2 mg vial + 1 mL BAC water = 2 mg/mL (20 units per 2 mg dose)
- 5 mg vial + 5 mL BAC water = 1 mg/mL (40 units per 2 mg dose, 100 units per 5 mg dose)
- 5 mg vial + 2.5 mL BAC water = 2 mg/mL (20 units per 2 mg dose, 50 units per 5 mg dose)
- 5 mg vial + 1 mL BAC water = 5 mg/mL (20 units per 5 mg dose)
Step-by-Step Reconstitution
- Wipe the top of both vials with alcohol swabs and allow to air dry.
- Draw your chosen BAC water volume into the syringe.
- Insert the needle into the TB-500 vial at a 45-degree angle.
- Inject the BAC water slowly down the inner glass wall, not directly onto the powder.
- Remove the needle and swirl gently until the powder is fully dissolved. Do not shake.
- The solution should be clear and colorless. Discard if cloudy or discolored.
- Label the vial with the reconstitution date and concentration (e.g., "1 mg/mL, 2026-05-18").
TB-500 Research Dosing Protocol
Animal and preclinical research protocols for TB-500 vary, but two general phases appear in the literature: a loading phase with higher weekly doses and a maintenance phase at lower doses. These reflect the protocols used in animal injury models.
Loading Phase (Weeks 1-6)
- Common range: 5-10 mg per week
- Often split into two injections: e.g., 2.5 mg twice weekly
- Subcutaneous injection is most documented in animal models
Maintenance Phase (Weeks 7+)
- Common range: 2-2.5 mg per week
- Frequency: once or twice weekly
- Duration in animal models: 4-8 additional weeks
Using the PeptiTools Calculator for TB-500
Open the PeptiTools TB-500 calculator and enter three values: your vial size in milligrams, how much BAC water you added in milliliters, and your dose in milligrams. The calculator outputs the exact unit number to draw on your U-100 insulin syringe, with a live diagram showing the fill line on the barrel.
Example: 5 mg vial, 5 mL BAC water, 2.5 mg dose. Concentration = 1 mg/mL. Draw = 2.5 mL = 250 units. That exceeds any standard syringe. Adjust: use 2 mg/mL instead (2.5 mL BAC water), and 2.5 mg now requires 1.25 mL = 125 units, still over a 1 mL syringe. Better: split into two 1.25 mg injections at 2 mg/mL = 62.5 units each, or use 5 mg/mL (1 mL BAC water) for 50 units per 2.5 mg dose.
TB-500 and BPC-157: Stacking Protocol
TB-500 and BPC-157 are frequently combined in preclinical research because they appear to work through complementary mechanisms. BPC-157 is thought to modulate growth factor signaling and promote tendon and gut healing, while TB-500 promotes cell migration, angiogenesis, and broader tissue repair via the actin-binding pathway. In animal studies, the combination is administered as separate injections, not mixed in the same vial.
- Reconstitute each peptide separately in its own vial
- Calculate draw amounts independently using PeptiTools for each
- Administer as separate injections at the same or different sites
- Never mix peptides in a single vial: concentration errors and stability interactions are unpredictable
Storage and Stability
Reconstituted TB-500 should be stored immediately in the refrigerator at 2-8°C. It is generally considered stable for up to 28 days when refrigerated, similar to GLP-1 peptides. Lyophilized TB-500 powder stored at -20°C remains stable for longer periods. Always check for cloudiness or color change before each injection and discard if the solution appears degraded.