BPC-157 (Body Protection Compound-157) is a synthetic pentadecapeptide of 15 amino acids, derived from a protective protein found in human gastric juice. It comes as a lyophilized white powder and must be reconstituted with bacteriostatic water before injection. Because it is dosed in micrograms rather than milligrams, precise concentration math is especially important.
What You Need
- BPC-157 vial (typically 2 mg or 5 mg)
- Bacteriostatic water (BAC water) for injection
- U-100 insulin syringes (0.3 mL or 0.5 mL, 28-31 gauge)
- Alcohol swabs
- Refrigerator set to 36-46°F (2-8°C)
Choosing Your BAC Water Volume and Concentration
The most common target concentration for BPC-157 is 500 mcg/mL (0.5 mg/mL) or 1000 mcg/mL (1 mg/mL). At 500 mcg/mL, a 250 mcg dose requires drawing 25 units on a U-100 syringe, which is a comfortable, easy-to-read amount. At 1000 mcg/mL, the same dose requires only 12.5 units, which is harder to measure accurately.
For most research protocols, 500 mcg/mL is the preferred concentration because it gives larger, more precise draw volumes for the typical 200-500 mcg dose range.
- 2 mg vial + 4 mL BAC water = 500 mcg/mL (25 units per 250 mcg dose)
- 2 mg vial + 2 mL BAC water = 1000 mcg/mL (12.5 units per 250 mcg dose)
- 5 mg vial + 10 mL BAC water = 500 mcg/mL (25 units per 250 mcg dose)
- 5 mg vial + 5 mL BAC water = 1000 mcg/mL (12.5 units per 250 mcg dose)
Step-by-Step Reconstitution
- Wipe the top of the BPC-157 vial and the BAC water vial with alcohol swabs. Allow to air dry.
- Draw the appropriate volume of BAC water into your insulin syringe.
- Insert the needle into the BPC-157 vial at a 45-degree angle.
- Inject the BAC water slowly down the inner glass wall of the vial. Do not aim directly at the powder.
- Remove the syringe and gently swirl the vial until the powder is fully dissolved. Never shake.
- The solution should be clear and colorless. Discard if you see cloudiness, color, or floating particles.
- Label the vial with the date reconstituted and the concentration (e.g., "500 mcg/mL, reconstituted 2026-05-14").
BPC-157 Research Dosing Protocol
Research protocols for BPC-157 typically use 200-500 mcg per injection, administered once or twice daily. Subcutaneous injection is the most commonly studied route in animal research, though intramuscular injection near the site of injury is also documented in preclinical studies. Oral administration has lower bioavailability and is outside the scope of this reconstitution guide.
- Conservative starting dose: 200 mcg per day
- Standard research dose: 250-500 mcg per day
- Common split dosing: 250 mcg morning, 250 mcg evening
- Typical cycle length: 4-12 weeks in animal models
Using the PeptiTools Calculator for BPC-157
Open the BPC-157 calculator on PeptiTools and enter your vial size (mg), the volume of BAC water you added (mL), and your desired dose (mcg). The calculator shows the exact number of units to draw on your syringe and displays a live syringe diagram marking that position on the barrel.
Example: 5 mg vial, 10 mL BAC water, 250 mcg dose. Concentration = 500 mcg/mL. Draw = 0.5 mL = 50 units on a U-100 syringe. The diagram will mark the 50-unit line on the barrel.
BPC-157 and TB-500: Reconstitution When Stacking
BPC-157 is frequently used alongside TB-500 (Thymosin Beta-4 fragment) in preclinical research. They are not mixed in the same vial. Each is reconstituted separately, stored separately, and injected as separate administrations. Use the PeptiTools calculator independently for each compound to get accurate draw amounts for both.
Storage and Stability
Once reconstituted, BPC-157 should be stored immediately in the refrigerator at 2-8°C. It is stable for approximately 7-14 days when refrigerated, which is shorter than GLP-1 peptides like semaglutide. Lyophilized (unmixed) BPC-157 powder can be stored at -20°C for longer-term stability.
Because of the shorter post-reconstitution window, many researchers reconstitute smaller volumes more frequently rather than preparing a large batch. A 2 mg vial reconstituted to 500 mcg/mL provides 4 mL of solution, which at 500 mcg/day lasts 8 days, fitting neatly within the stability window.