How to Reconstitute Hexarelin: Vial Math and Dosing

Hexarelin ships as a lyophilized powder, usually in 2 mg or 5 mg vials, and has to be reconstituted with bacteriostatic water before the first injection. The math matters more here than with a mild secretagogue: typical hexarelin doses sit at 100 to 200 mcg, and the difference between those two numbers is the difference between modest cortisol and prolactin spillover and clinically noticeable spillover. A concentration you can read off an insulin syringe without arithmetic is the practical goal.

What You Need Before You Start

• Hexarelin vial, typically 2 mg or 5 mg lyophilized powder
• Bacteriostatic water (0.9% benzyl alcohol), not plain sterile water
• A 1 mL U-100 insulin syringe (28 to 31 gauge) for injection
• A 3 mL syringe with a longer needle for transferring BAC water, optional but easier
• Alcohol swabs and a refrigerator set to 2 to 8°C (36 to 46°F)

Choosing Your BAC Water Volume

The default for a 2 mg hexarelin vial is 2 mL of bacteriostatic water. That yields 1 mg/mL, which is 1000 mcg/mL, so 100 mcg is exactly 10 units on a U-100 syringe and 200 mcg is 20 units. There is no conversion to memorize and no rounding error to introduce at the point of injection.

For a 5 mg vial, 2.5 mL of BAC water gives 2 mg/mL (2000 mcg/mL), where 100 mcg is 5 units and 200 mcg is 10 units. That is workable, but it halves your resolution: a one-unit misread is now a 20 mcg error rather than 10 mcg. If you are dosing at the low end or titrating carefully, 5 mL into a 5 mg vial (back to 1000 mcg/mL) is the more forgiving choice, provided the vial has the headroom to hold it.

Step-by-Step Reconstitution

1. Let the hexarelin vial come to room temperature if it was refrigerated or shipped cold. Cold glass plus rapid dilution encourages the powder to clump.
2. Swab the rubber stopper on both the hexarelin vial and the BAC water vial with alcohol and let them air dry.
3. Draw your chosen volume of bacteriostatic water into the syringe, for example 2 mL for a 2 mg vial.
4. Insert the needle into the hexarelin vial at roughly 45 degrees and aim the tip at the inner glass wall, not at the powder cake.
5. Release the plunger slowly and let the vacuum in the vial pull the water down the wall. Forcing it in as a jet shears the peptide.
6. Withdraw the needle, then roll or swirl the vial gently between your fingers until the solution is clear. Do not shake it.
7. Inspect against a light source. The solution should be clear and colorless with no visible particulates, fibers, or cloudiness.
8. Write the reconstitution date and the final concentration in mcg/mL directly on the vial label, then refrigerate.

Full dissolution normally takes under a minute of gentle swirling. If flakes are still visible after two or three minutes, leave the vial upright in the fridge for 15 minutes and swirl again rather than agitating it harder.

Concentration Reference Table

The four combinations below cover almost every hexarelin vial you will encounter. Units refer to a standard U-100 insulin syringe.

• 2 mg vial + 2 mL BAC water = 1000 mcg/mL: 100 mcg is 10 units, 200 mcg is 20 units
• 2 mg vial + 1 mL BAC water = 2000 mcg/mL: 100 mcg is 5 units, 200 mcg is 10 units
• 5 mg vial + 5 mL BAC water = 1000 mcg/mL: 100 mcg is 10 units, 200 mcg is 20 units
• 5 mg vial + 2.5 mL BAC water = 2000 mcg/mL: 100 mcg is 5 units, 200 mcg is 10 units

If your vial size or BAC volume is not on this list, put the numbers into the hexarelin calculator and it will return the exact unit mark with a live syringe diagram. The general reconstitution calculator handles any vial and concentration combination.

How Many Doses Per Vial

A 2 mg vial holds 2000 mcg of peptide. At 100 mcg per injection that is 20 doses, and at 200 mcg it is 10 doses. Running twice daily at 100 mcg, a 2 mg vial lasts 10 days, which sits comfortably inside the 28-day refrigerated window. A 5 mg vial at 200 mcg twice daily gives 12 to 13 days of use, also within the window. Plan your vial size around your dosing cadence so you are not discarding half a vial at day 28.

Storage After Reconstitution

Refrigerate reconstituted hexarelin at 2 to 8°C immediately after mixing and keep it there between injections. With bacteriostatic water it is generally treated as stable for up to 28 days. Store the vial upright in the body of the fridge rather than the door, where temperature swings with every opening, and keep it out of direct light. Do not freeze reconstituted peptide: ice crystal formation and the freeze-thaw cycle degrade the peptide chain.

Unreconstituted lyophilized hexarelin is far more robust. Sealed vials of powder can be kept refrigerated for a year or more, and frozen for longer, which is the reason to reconstitute one vial at a time rather than the whole order at once.

Injection Timing and Fasted State

Hexarelin is injected subcutaneously, most often into abdominal fat two inches from the navel. Elevated blood glucose and insulin blunt the pituitary GH response, so injections are timed to fasted windows: first thing in the morning, and optionally before sleep at least three hours after the last meal. Wait 20 to 30 minutes after injecting before eating.

Hexarelin has a half-life of roughly 70 minutes, so each injection produces a discrete GH pulse rather than a sustained elevation. That is why cadence (one to three injections daily) matters more than total daily milligrams for this class of compound.

Desensitization: Why the Dose You Pick Matters

Hexarelin desensitizes the pituitary faster than ipamorelin or GHRP-2. Measurable attenuation begins after 2 to 3 weeks of twice-daily dosing, and continuous use at 200 mcg twice daily typically reduces the GH response by around 50% by week 4 to 6. Standard practice is 8 to 12 weeks on followed by a 4-week break, holding 100 mcg once daily during the maintenance phase rather than escalating.

The most effective way to keep the dose low is to stack a GHRH analog. Pairing 100 mcg hexarelin with 100 mcg CJC-1295 without DAC produces a GH pulse comparable to 200 mcg hexarelin alone, with proportionally less cortisol and prolactin spillover. Both peptides can be drawn into the same syringe and injected together.